Product Pathways - Glucose Metabolism
PKM1/2 Antibody #3186
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R (C) (X) | Endogenous | 60 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PKM1/2 Antibody detects endogenous levels of total PKM (including M1 and M2) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human PKM. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using PKM1/2 Antibody.
Background
Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, four different isozymes of this kinase have been identified: M1, M2, L and R. M1 is the major form in brain and muscle, and M2 is found in early stage fetal cells and most adult tissues. L and R are present in the liver and red blood cells, respectively. Tumor cells usually overexpress M2 isozyme (PKM2), leading to an increase of the amount of glycolytic phospho-metabolites in these cells (1). This isozyme can exist in a tetrameric form with a high substrate affinity or a dimeric form with a low affinity for its substrate (2). In tumor cells, the dimeric form is generally predominant (2). Studies suggest that this isozyme could potentially serve as a marker for gastric and colorectal tumors (1,2)
- Zhang, B. et al. (2004) World J. Gastroenterol. 10, 1643-1646.
- Hardt, P.D. et al. (2004) Br. J. Cancer 91, 980-984.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
