Product Pathways - Lymphocyte Signaling
NQO1 (A180) Mouse mAb #3187
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC | H | Endogenous | 29 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
NQO1 (A180) Mouse mAb detects endogenous levels of total NQO1 protein.
Source / Purification
NQO1 (A180) Mouse mAb is produced by immunizing mice with purified recombinant human NQO1.
Western Blotting
Western blot analysis of extracts from several cell lines, using NQO1 (A180) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using NQO1 (A180) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using NQO1 (A180) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using NQO1 (A180) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa (left) and NIH/3T3 cells (right) labeled with NQO1 (A180) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoprotein that catalyzes the two-electron reduction of quinones and their derivatives (1,2). This enzyme protects cells against redox cycling and oxidative stress (1,3). The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues (1,2). Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development (1). Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turns leads to myeloid hyperplasia of bone marrow (2).
- Belinsky, M. and Jaiswal, A.K. (1993) Cancer Metastasis Rev. 12, 103-117.
- Siegel, D. et al. (1998) Clin. Cancer Res. 4, 2065-2070.
- Long, D.J. et al. (2002) Cancer Res. 62, 3030-3036.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
