Cell Signaling Technology

Product Pathways - Metabolism

Aldolase A Antibody #3188

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk Endogenous 40 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Aldolase A Antibody detects endogenous levels of total fructose bisphosphate aldolase A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of humanfructose bisphosphate aldolase A protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using Aldolase A Antibody.

Background

Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Research studies suggest that aldolase A expression potentially differentiates between nonneoplastic liver diseases and hepatocarcinoma (1). Furthermore, investigators have shown that changes in aldolase B gene expression levels have been observed in certain patients with this primary tumor (2,3).

  1. Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
  2. Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
  3. Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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