Product Pathways - Glucose Metabolism
Aldolase Antibody #3188
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk Hm | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Hm=Hamster
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Aldolase Antibody detects endogenous levels of total fructose bisphosphate aldolase (including aldolases A, B and C) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of humanfructose bisphosphate aldolase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Aldolase Antibody.
Background
Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B and aldolase C. Studies suggest the potential diagnostic value of quantitive anlysis of aldolase A for hepatocarcinoma (1). Furthermore, the change of aldolase B gene expression levels has also been observed in certain patients with this primary tumor (2,3).
- Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
- Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
- Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
