Product Pathways - Glucose Metabolism
Aldolase Antibody #3188
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Hm Mk | Endogenous | 40 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3188:
- Western Blotting
Specificity / Sensitivity
Aldolase Antibody detects endogenous levels of total fructose bisphosphate aldolase (including aldolases A, B and C) protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of humanfructose bisphosphate aldolase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Aldolase Antibody.
Background
Aldolase (fructose bisphosphate aldolase), a glycolytic enzyme, catalyzes the conversion of fructose 1, 6-bisphosphate to 3-phosphoglyceraldehyde. This ubiquitous enzyme is present as three different isozymes: aldolase A, aldolase B, and aldolase C. Studies suggest the potential diagnostic value of quantitative analysis of aldolase A for hepatocarcinoma (1). Furthermore, the change in aldolase B gene expression levels has also been observed in certain patients with this primary tumor (2,3).
- Castaldo, G. et al. (2000) Clin. Chem. 46, 901-906.
- Kinoshita, M. and Miyata, M. (2002) Hepatology 36, 433-438.
- Song, H. et al. (2004) World J. Gastroenterol 10, 509-513.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3186 PKM1/2 Antibody
- 2118 GAPDH (14C10) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
