Product Pathways - Glucose Metabolism
Fatty Acid Synthase Antibody #3189
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M | Endogenous | 273 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Fatty Acid Synthase Antibody detects endogenous levels of total fatty acid synthase protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) around Ala1160 derived from a sequence of mouse fatty acid synthase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Fatty Acid Synthase Antibody.
Background
Fatty acid synthase (FAS) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FAS is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FAS is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1).More recently, increased expression of FAS has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FAS are directly related to the size of breast tumors (2). Studies also showed that FAS is highly expressed in lung and prostate cancers and that FAS expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FAS is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FAS as a potential target for the diagnosis and treatment of cancer as well as the metabolic syndrome (6,7).
- Katsurada, A. et al. (1990) Eur J Biochem 190, 427-33.
- Wells, W.A. et al. (2006) Breast Cancer Res Treat 98, 231-40.
- Kawamura, T. et al. (2005) Pathobiology 72, 233-240.
- Shah, U.S. et al. (2006) Hum Pathol 37, 401-409.
- Kuhajda, F.P. (2000) Nutrition 16, 202-8.
- Tian, W.X. (2006) Curr Med Chem 13, 967-977.
- Kusunoki, J. et al. (2006) Endocrine 29, 91-100.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
