Product Pathways - Glucose Metabolism
PKM1/2 (C103A3) Rabbit mAb #3190
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 60 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
PKM1/2 (C103A3) Rabbit mAb detects endogenous levels of total PKM (including M1 and M2) protein.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence around Gly200 of human PKM2.
Western Blotting
Western blot analysis of extracts from various cell types using PKM1/2 (C103A3) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using PKM1/2 (C103A3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Pyruvate kinase, a glycolytic enzyme, catalyses the conversion of phosphoenolpyruvate to pyruvate. In mammals, the M1 isoform (PKM1) is expressed in most adult tissues (1). The M2 isoform (PKM2), an alternatively-spliced variant of M1, is expressed during embryonic development (1). Studies found that cancer cells exclusively express PKM2 (1, 2, 3). PKM2 is shown to be essential for aerobic glycolysis in tumors (Warburg effect) (1). When the M2 isoform is switched to the M1 isoform, aerobic glycolysis is reduced and oxidative phosphorylation is increased in cancer cells (1). These cells also show decreased tumorigenicity in mouse xenografts (1).
- Christofk, H.R. et al. (2008) Nature 452, 230-3.
- Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8.
- Dombrauckas, J.D. et al. (2005) Biochemistry 44, 9417-29.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
