Product Pathways - Adhesion
E-Cadherin (24E10) Rabbit mAb #3195
|W IHC-P IHC-F IF-IC F||H M (B) (Dg) (Pg)||Endogenous||135||Rabbit IgG|
Reactivity Key: H=Human M=Mouse B=Bovine Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Pro780 of human E-cadherin protein.
Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).
Immunohistochemical analysis of frozen HCC827 xenograft, showing membrane and cytoplasmic localization using E-Cadherin (24E10) Rabbit mAb.
Flow cytometric analysis of HeLa cells (blue) and MCF7 cells (green) using E-Cadherin (24E10) Rabbit mAb.
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
- Christofori, G. (2003) EMBO J. 22, 2318-2323.
- Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163.
- Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434.
- Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377.
- Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997.
- Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177.
- Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.
- Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting
- Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting
- Chow, G. et al. (2010) J Biomed Biotechnol 2010, 485468. Applications: IF-IC (In Cells)
- Kroening, S. et al. (2010) Am J Physiol Renal Physiol 298, F796-806. Applications: Western Blotting
- Kong, B. et al. (2010) Oncogene 29, 5146-58. Applications: Western Blotting
- Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: IF-IC (In Cells)
- Tauler, J. et al. (2010) Cancer Res 70, 7137-47. Applications: IF-IC (In Cells) Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and7,429,487) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.