Product Pathways - Adhesion

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E-Cadherin (24E10) Rabbit mAb #3195

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IHC-F IF-IC F	H M (Dg) (Pg)	Endogenous	135	Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  Dg=Dog  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3195:

Flow, IHC / Frozen, IHC / Paraffin, Immunofluorescence, Western Blotting

Specificity / Sensitivity

E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Pro780 of human E-cadherin protein.

Background

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235.
2. Christofori, G. (2003) EMBO J. 22, 2318-2323.
3. Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163.
4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434.
5. Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377.
6. Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997.
7. Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177.
8. Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.

Application References

• Qattan, A.T. et al. (2010) J Proteome Res 9, 495-508. Applications: Western Blotting
• Xiang, X. et al. (2011) PLoS One 6, e14640. Applications: Western Blotting
• Chow, G. et al. (2010) J Biomed Biotechnol 2010, 485468. Applications: IF-IC (In Cells)
• Kroening, S. et al. (2010) Am J Physiol Renal Physiol 298, F796-806. Applications: Western Blotting
• Kong, B. et al. (2010) Oncogene 29, 5146-58. Applications: Western Blotting
• Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: IF-IC (In Cells)

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 1056 E-Cadherin Blocking Peptide
• 3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor^® 488 Conjugate)
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)

Cell Signaling Technology^® is a trademark of Cell Signaling Technology, Inc.Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and7,429,487) from Epitomics, Inc.

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.