Product Pathways - Tyrosine Kinase/ Adaptors

Basic FGF (19A9) Rabbit mAb #3196

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Applications	Reactivity	MW (kDa)	Source	Isotype
W IP	H	19	Rabbit	IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Basic FGF (19A9) Rabbit mAb detects recombinant human basic FGF at various concentrations. It may cross-react with human acidic FGF proteins at high concentration levels.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with recombinant human basic FGF proteins.

Background

Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).

1. Powers, C.J. et al. (2000) Endocrine-Related Cancer 7, 165-197.
2. Bansal, R. (2002) Dev. Neurosci. 24, 35-46.
3. Morrison, R.S. et al. (1994) J. Neurooncol. 18, 207-216.
4. Kouhara, H. et al. (1997) Cell 89, 693-702.
5. Mohammadi, M. et al. (1991) Mol. Cell. Biol. 11, 5068-5078.
6. Bikfalvi, A. (1995) Eur. J. Cancer 31A, 1101-1104.
7. Ledoux, D. et al. (1992) Prog. Growth Factor Res. 4, 107-120.

Application References

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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.