Product Pathways - Tyrosine Kinase / Adaptors
Basic FGF (19A9) Rabbit mAb #3196
|3196S||100 µl (10 western blots)||---||In Stock||---|
|3196||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Basic FGF (19A9) Rabbit mAb detects recombinant human basic FGF at various concentrations. It may cross-react with human acidic FGF proteins at high concentration levels.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human basic FGF proteins.
Fibroblast growth factors are a family of broad-spectrum growth factors influencing a plethora of cellular activities. The interaction of at least 23 ligands, 4 receptors and multiple coreceptors provides a dramatic complexity to a signaling system capable of effecting a multitude of responses (1,2). Basic fibroblast growth factor (bFGF or FGF2), initially identified as a mitogen with prominent angiogenic properties, is now recognized as a multifunctional growth factor (3). It is clear that bFGF produces its biological effects in target cells by signaling through cell-surface FGF receptors. bFGF binds to all four FGF receptors. Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic downstream target proteins, including FRS-2, PLC and Crk (4,5). The FGF signaling pathway appears to play a significant role not only in normal cell growth regulation but also in tumor development and progression (6).
Acidic FGF (aFGF or FGF1) is another extensively investigated protein of the FGF family. aFGF shares 55% DNA sequence homology with bFGF. These two growth factors are ubiquitously expressed and exhibit a wide spectrum of similiar biological activities with quantitative differences likely due to variation in receptor affinity or binding (7).
- Powers, C.J. et al. (2000) Endocrine-Related Cancer 7, 165-197.
- Bansal, R. (2002) Dev. Neurosci. 24, 35-46.
- Morrison, R.S. et al. (1994) J. Neurooncol. 18, 207-216.
- Kouhara, H. et al. (1997) Cell 89, 693-702.
- Mohammadi, M. et al. (1991) Mol. Cell. Biol. 11, 5068-5078.
- Bikfalvi, A. (1995) Eur. J. Cancer 31A, 1101-1104.
- Ledoux, D. et al. (1992) Prog. Growth Factor Res. 4, 107-120.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3139 Acidic FGF (11H11) Rabbit mAb
- 3471 Phospho-FGF Receptor (Tyr653/654) Antibody
- 3472 FGF Receptor 1 Antibody
- 3476 Phospho-FGF Receptor (Tyr653/654) (55H2) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2544 Phospho-FGF Receptor 1 (Tyr766) (1E5) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.