Product Pathways - Neuroscience
SynGAP Antibody #3200
PhosphoSitePlus® protein, site, and accession data: SynGAP
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R (H) | Endogenous | 140 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3200:
- Western Blotting
Specificity / Sensitivity
SynGAP Antibody detects endogenous levels of total SynGAP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SynGAP. Antibodies are purified by peptide affinity chromatography.
Background
SynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ domains of SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123, among other sites (2,3). Phosphorylation of SynGAP results in stimulation of the GTPase activity of Ras, and PSD95 dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development as mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced long-term potentiation (LTP) and perform poorly in spatial memory tasks (6).
- Kim, J.H. et al. (1998) Neuron 20, 683-91.
- Oh, J.S. et al. (2004) J Biol Chem 279, 17980-8.
- Krapivinsky, G. et al. (2004) Neuron 43, 563-74.
- Song, B. et al. (2004) Brain Res 1005, 44-50.
- Komiyama, N.H. et al. (2002) J Neurosci 22, 9721-32.
- Kim, J.H. et al. (2003) J Neurosci 23, 1119-24.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3339 Ras (27H5) Rabbit mAb
- 2507 PSD95 Antibody
- 3381 Phospho-NMDAR1 (Ser890) Antibody
- 4205 NMDAR2A Antibody
- 3362 CaMKII (pan) Antibody
- 3361 Phospho-CaMKII (Thr286) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.