Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Yes Antibody #3201

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk B Endogenous 60 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Yes Antibody detects endogenous levels of total Yes protein. This antibody does not cross-react with Src.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Yes protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Yes Antibody.

Background

The cellular oncogene c-Yes and its viral homologue v-Yes (the transforming gene of Yamaguchi 73 and Esh avian sarcoma viruses) encode a 60 kDa, cytoplasmic, membrane-associated, protein-tyrosine kinase (1). Yes belongs to the Src kinase family and is ubiquitously expressed in many tissues and cells. Like other Src family members, Yes contains several conserved functional domains such as an N-terminal myristoylation sequence for membrane targeting, SH2 and SH3 domains, a kinase domain, and a C-terminal non-catalytic domain (2). Although several lines of evidence support redundancy in signaling between Yes and other Src family kinases, there is also a growing body of evidence indicating specificity in Yes signaling (2). Yes is activated downstream of a multitude of cell surface receptors, including receptor tyrosine kinases, G protein-coupled receptors, and cytokine receptors (3). In addition, both Yes and Src kinases are activated during the cell cycle transition from G2 to M phase (3). Investigators have found that dysfunction of Yes is associated with the development of various cancers (4).

  1. Pena, S.V. et al. (1995) Gastroenterology 108, 117-24.
  2. Summy, J.M. et al. (2003) Front Biosci 8, s185-205.
  3. Summy, J.M. et al. (2003) J Cell Sci 116, 2585-98.
  4. Rungsipipat, A. et al. (1999) Res Vet Sci 66, 205-10.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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