Product Pathways - Glucose Metabolism
Pyruvate Dehydrogenase (C54G1) Rabbit mAb #3205
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P | H M R Mk | Endogenous | 43 | Rabbit igG |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 3205:
- IHC / Paraffin, Western Blotting
Specificity / Sensitivity
Pyruvate Dehydrogenase (C54G1) Rabbit mAb detects endogenous levels of total pyruvate dehydrogenase α1 subunit.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human pyruvate dehydrogenase.
Western Blotting
Western blot analysis of extracts from various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
Western Blotting
Western blot analysis of various cell lines using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Pyruvate Dehydrogenase (C54G1) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Background
The pyruvate dehydrogenase complex catalyzes the conversion of pyruvate and CoA into acetyl-CoA and CO2 in the presence of NAD+. Acetyl-CoA then goes into the citric acid cycle where it reacts with oxaloacetate to form citrate. Acetyl-CoA is also used for fatty acid and cholesterol biosynthesis. The reaction of oxidative decarboxylation of pyruvate therefore serves as a critical link between glycolysis and the citric acid cycle and lipid metabolism. In mammalian cells, the pyruvate dehydrogenase complex is located in the mitochondrial matrix (1). This complex is comprised of three enzymes: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). Pyruvate dehydrogenase (E1) consists of two subunits: α and β. This enzyme catalyzes the removal of CO2 from pyruvate. Mutations in the α subunits of pyruvate dehydrogenase (E1) lead to congenital defects that are usually associated with lactic acidosis, neurodegeneration and early death (2).
- Strumiło, S. (2005) Acta Biochim Pol 52, 759-64.
- Stacpoole, P.W. et al. (2003) Curr Gene Ther 3, 239-45.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
