Product Pathways - Lymphocyte Signaling
GPX1 Antibody #3206
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 22 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GPX1 Antibody detects endogenous levels of total GPX1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human GPX1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293 and THP1 cells, using GPX1 Antibody.
Background
Glutathione peroxidase 1 (GPX1) is a cytosolic selenoprotein which reduces hydrogen peroxide to water (1). GPX1 is the most abundant and ubiquitous among the five GPX isoforms identified so far (2). It is an important component in the anti-oxidative defense in cells and is associated with a variety of disease conditions, such as colon cancer (3), coronary artery disease (4) and insulin resistance (1).
- McClung, J.P. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 8852-8857.
- Hamanishi, T. et al. (2004) Diabetes 53, 2455-2460.
- Drew, J.E. et al. (2005) FEBS Lett. 579, 6135-6139.
- Winter, J.P. et al. (2003) Coron. Artery Dis. 14, 149-153.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
