Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

GPX1 Antibody #3206

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 22 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GPX1 Antibody detects endogenous levels of total GPX1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GPX1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and THP1 cells, using GPX1 Antibody.

Background

Glutathione peroxidase 1 (GPX1) is a cytosolic selenoprotein which reduces hydrogen peroxide to water (1). GPX1 is the most abundant and ubiquitous among the five GPX isoforms identified so far (2). It is an important component in the anti-oxidative defense in cells and is associated with a variety of disease conditions, such as colon cancer (3), coronary artery disease (4) and insulin resistance (1).

  1. McClung, J.P. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 8852-8857.
  2. Hamanishi, T. et al. (2004) Diabetes 53, 2455-2460.
  3. Drew, J.E. et al. (2005) FEBS Lett. 579, 6135-6139.
  4. Winter, J.P. et al. (2003) Coron. Artery Dis. 14, 149-153.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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