Cell Signaling Technology

Product Pathways - Metabolism

AMACR (2A10) Mouse mAb #3207

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 42 Mouse IgG2b

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AMACR (2A10) Mouse mAb detects endogenous levels of total AMACR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a purified truncated recombinant human AMACR-His fusion protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP cells using AMACR (2A10) Mouse mAb.

Background

α-methylacyl-CoA racemase (AMACR), an enzyme localized in peroxisomes and mitochondria, is involved in the β-oxidation of branched-chain fatty acids and fatty acid derivatives (1). AMACR has been reported to be a biomarker for prostate cancer (2-4). The expression of AMACR is also related to other types of cancers such as hepatocellular carcinoma (1), noninvasive bladder cancer (5), colorectal cancer (6) and gastric adenocarcinoma (7).

  1. Li, W. et al. (2008) J Exp Clin Cancer Res 27, 2.
  2. Rubin, M.A. et al. (2002) JAMA 287, 1662-70.
  3. Jiang, Z. et al. (2002) Am J Surg Pathol 26, 1169-74.
  4. Jiang, Z. et al. (2001) Am J Surg Pathol 25, 1397-404.
  5. Gunia, S. et al. (2008) Virchows Arch 453, 165-70.
  6. Marx, A. et al. (2008) Virchows Arch 453, 243-248.
  7. Truong, C.D. et al. (2008) Int J Clin Exp Pathol 1, 518-523.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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