Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

AP-2α Antibody #3208

Applications Reactivity Sensitivity MW (kDa) Source
W IP IHC-P H M R Mk Endogenous 48 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

AP-2α Antibody detects endogenous levels of total AP-2α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human AP-2α. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines, using AP-2α Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AP-2alpha Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using AP-2alpha Antibody.


Background

The sequence-specific transcription factor activator protein 2α (AP-2α) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2α expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2α expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2α expression. Furthermore, p53 binds to the cis-element in the AP-2α promoter, suggesting that AP-2α is a target of p53. AP-2α may mediate the effect of p53 to inhibit cell proliferation (8).

  1. Mitchell, P.J. et al. (1987) Cell 50, 847-61.
  2. Williams, T. and Tjian, R. (1991) Genes Dev 5, 670-82.
  3. Douglas, D.B. et al. (2004) Cancer Res 64, 1611-20.
  4. Gee, J.M. et al. (1999) J Pathol 189, 514-20.
  5. Anttila, M.A. et al. (2000) Br J Cancer 82, 1974-83.
  6. Jean, D. et al. (1998) J Biol Chem 273, 16501-8.
  7. Ruiz, M. et al. (2004) Cancer Res 64, 631-8.
  8. Li, H. et al. (2006) Oncogene 25, 5405-15.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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