Product Pathways - Apoptosis
AP-2α (C83E10) Rabbit mAb #3215
PhosphoSitePlus® protein, site, and accession data: AP-2 alpha
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 48 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
AP-2α (C83E10) Rabbit mAb detects endogenous levels of total AP-2α protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human AP-2α.
Western Blotting
Western blot analysis of extracts from various cell types using AP-2α (C83E10) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using AP-2α (C83E10) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
The sequence-specific transcription factor activator protein 2α (AP-2α) is required for normal growth and morphogenesis during mammalian development (1,2). Decreased or loss of AP-2α expression has been observed in many different types of human cancers including breast cancer (3,4), ovarian cancer (5), melanoma (6) and prostate cancer (7). These findings suggest that AP-2α expression plays a crucial role in tumorigenicity. Studies have also shown that p53 overexpression in human breast carcinoma cells induces the level of AP-2α expression. Furthermore, p53 binds to the cis-element in the AP-2α promoter, suggesting that AP-2α is a target of p53. AP-2α may mediate the effect of p53 to inhibit cell proliferation (8).
- Mitchell, P.J. et al. (1987) Cell 50, 847-61.
- Williams, T. and Tjian, R. (1991) Genes Dev 5, 670-82.
- Douglas, D.B. et al. (2004) Cancer Res 64, 1611-20.
- Gee, J.M. et al. (1999) J Pathol 189, 514-20.
- Anttila, M.A. et al. (2000) Br J Cancer 82, 1974-83.
- Jean, D. et al. (1998) J Biol Chem 273, 16501-8.
- Ruiz, M. et al. (2004) Cancer Res 64, 631-8.
- Li, H. et al. (2006) Oncogene 25, 5405-15.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3208 AP-2α Antibody
- 2509 AP-2β Antibody
- 2320 AP-2γ Antibody
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
