Cell Signaling Technology

Product Pathways - Development

Dvl3 Antibody #3218

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Hm Mk Mi B Endogenous 88 to 93 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Mi=Mink  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dvl3 Antibody detects endogenous levels of total Dvl3 protein. This antibody does not cross-react with Dvl2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3. The antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3, C6, A549, BAEC and COS cells using Dvl3 Antibody.

Background

Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8).

  1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
  2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
  3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
  4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
  5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
  6. Chen, W. et al. (2003) Science 301, 1391-1394.
  7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
  8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products