Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Ret Antibody #3220

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 170, 175 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ret Antibody detects endogenous levels of total Ret protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1085 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from TT cells using Ret Antibody.

Background

The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5).

  1. Airaksinen, M.S. et al. (1999) Mol. Cell. Neurosci. 13, 313-325.
  2. Takahashi, M. et al. (1989) Oncogene 4, 805-806.
  3. Manie, S. et al. (2001) Trends Genet. 17, 580-589.
  4. Tallini, G. and Asa, S. (2001) Adv. Anat. Pathol. 8, 345-354.
  5. Iwashita, T. et al. (1999) Oncogene 18, 3919-3922.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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