Product Pathways - Tyrosine Kinase / Adaptors
Phospho-Ret (Tyr905) Antibody #3221
|3221S||100 µl (10 western blots)||---||In Stock||---|
|3221||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, D. melanogaster||Endogenous||175||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905. This antibody may cross-react with other activated receptor tyrosine kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of recombinant human GST-Ret (His658-Asp1110) and extracts from the TT human medullary thyroid carcinoma cell line using Phospho-Ret (Tyr905) Antibody.
The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5).
- Mologni, L. et al. (2006) J Mol Endocrinol 37, 199-212. Applications: Western Blotting.
- Strock, C. J. et al. (2003) . Cancer Res. 63, 5559-5563. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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