Product Pathways - Tyrosine Kinase / Adaptors
Phospho-Ret (Tyr905) Antibody #3221
PhosphoSitePlus® protein, site, and accession data: Ret
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H Dm | Endogenous | 175 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Dm=D. melanogaster
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Phospho-Ret (Tyr905) Antibody detects endogenous levels of Ret only when phosphorylated at tyrosine 905. This antibody may cross-react with other activated receptor tyrosine kinases.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of recombinant human GST-Ret (His658-Asp1110) and extracts from the TT human medullary thyroid carcinoma cell line using Phospho-Ret (Tyr905) Antibody.
Western Blotting
Western blot analysis of recombinant human GST-Ret (His658-Asp1110), untreated or λ phosphatase-treated, using Phospho-Ret (Tyr905) Antibody (upper) and Ret Antibody #3220 (lower).
Background
The Ret proto-oncogene (c-Ret) is a receptor tyrosine kinase that functions as a multicompetent receptor complex in conjunction with other membrane-bound, ligand-binding GDNF family receptors (1). Ligands that bind the Ret receptor include the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin, and artemin (2-4). Research studies have shown that alterations in the corresponding RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung’s disease (1,3). The Tyr905 residue located in the Ret kinase domain plays a crucial role in Ret catalytic and biological activity. Substitution of Phe for Tyr at position 905 dramatically inhibits Ret autophosphorylation activity (5).
- Airaksinen, M.S. et al. (1999) Mol. Cell. Neurosci. 13, 313-325.
- Takahashi, M. et al. (1989) Oncogene 4, 805-806.
- Manie, S. et al. (2001) Trends Genet. 17, 580-589.
- Tallini, G. and Asa, S. (2001) Adv. Anat. Pathol. 8, 345-354.
- Iwashita, T. et al. (1999) Oncogene 18, 3919-3922.
Application References
- Mologni, L. et al. (2006) J Mol Endocrinol 37, 199-212. Applications: Western Blotting
- Strock, C. J. et al. (2003) CEP-701 and CEP-751 Inhibit Constitutively Activated RET Tyrosine Kinase Activity and Block Medullary Thyroid Carcinoma Cell Growth. Cancer Res. 63, 5559-5563. Applications: Western Blotting
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Companion Products
- 3223 Ret (C31B4) Rabbit mAb
- 3220 Ret Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
