Cell Signaling Technology

Product Pathways - Development

Dvl2 (30D2) Rabbit mAb #3224

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 90 to 95 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Dvl2 (30D2) Rabbit mAb detects endogenous levels of total Dvl2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly176 of human Dvl2.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from various cell lines using Dvl2 (30D2) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from Rat2 and NIH/3T3 cells, untreated or treated with conditioned medium containing Wnt3a for 60 minutes and also treated with or without calf intestinal phosphatase (CIP), using Dvl2 (30D2) Rabbit mAb.

Background

Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8).

  1. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810.
  2. Boutros, M. and Mlodzik, M. (1999) Mech. Dev. 83, 27-37.
  3. Lee, J.S. et al. (1999) J. Biol. Chem. 274, 21464-70.
  4. Itoh, K. et al. (2005) J. Biol. 4, 3.1-3.12.
  5. Capelluto, D.G. et al. (2002) Nature 419, 726-729.
  6. Chen, W. et al. (2003) Science 301, 1391-1394.
  7. Okino , K. et al. (2003) Oncol. Rep. 10, 1219-1223.
  8. Uematsu, K. et al. (2003) Oncogene 22, 7218-7221.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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