Cell Signaling Technology

Product Pathways - MAPK Signaling

TAB1 (C25E9) Rabbit mAb #3226

Applications Reactivity MW (kDa) Source Isotype
W IP H Mk 60 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

TAB1 (C25E9) Rabbit mAb detects endogenous levels of total TAB1 protein.

Source / Purification

Rabbit monoclonal antibodies were prepared from spleens of rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro381 of human TAB1.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat, HeLa and Caki cell lines using TAB1 (C25E9) Rabbit mAb.

Background

TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-β and various pro-inflammatory signals (1,2). In vivo TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK respectively. TAK1 and TRAF6 also activate the NF-κB pathway by phosphorylating the NF-κB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38α (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8).

  1. Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
  2. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
  3. Shibuya, H. et al. (1996) Science 272, 1179-1182.
  4. Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
  5. Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
  6. Wang, C. et al. (2001) Nature 412, 346-351.
  7. Ge, B. et al. (2002) Science 295, 1291-1294.
  8. Komatsu, Y. et al. (2002) Mech. Dev. 119, 239-249.

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