Product Pathways - MAPK Signaling
TAB1 (C25E9) Rabbit mAb #3226
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H Mk | 60 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TAB1 (C25E9) Rabbit mAb detects endogenous levels of total TAB1 protein.
Source / Purification
Rabbit monoclonal antibodies were prepared from spleens of rabbits immunized with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro381 of human TAB1.
Background
TAK1 is a mitogen-activated protein kinase kinase kinase activated by TGF-β and various pro-inflammatory signals (1,2). In vivo TAK1 activation requires its association with TAK1 binding protein 1 (TAB1), which triggers TAK1 autophosphorylation at Thr184 and Thr187 (3,4). The TAB2 adaptor protein links TAK1 with TRAF6 to mediate TAK1 activation following IL-1 stimulation (5). Once activated, TAK1 phosphorylates MAPK kinases MKK4 and MKK3/6, which activate p38 MAPK and JNK respectively. TAK1 and TRAF6 also activate the NF-κB pathway by phosphorylating the NF-κB inducing kinase (NIK) to trigger subsequent activation of IKK (2,6). In addition to TAK1, TAB1 interacts with and activates p38α (7). Targeted disruption of the TAB1 gene in mice causes a drastic reduction in TAK1 activity and leads to embryonic lethality (8).
- Yamaguchi, K. et al. (1995) Science 270, 2008-2011.
- Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-256.
- Shibuya, H. et al. (1996) Science 272, 1179-1182.
- Sakurai, H. et al. (2000) FEBS Lett. 474, 141-145.
- Takaesu, G. et al. (2000) Mol. Cell 4, 649-658.
- Wang, C. et al. (2001) Nature 412, 346-351.
- Ge, B. et al. (2002) Science 295, 1291-1294.
- Komatsu, Y. et al. (2002) Mech. Dev. 119, 239-249.
Application References
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