Product Pathways - Translational Control
GβL Antibody #3227
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P | H | Endogenous | 37 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
GβL Antibody detects endogenous levels of total GβL protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using GβL Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using GβL Antibody in the presence of control peptide (left) or antigen specific peptide (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using GβL Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, using GβL Antibody.
Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
- Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3274 GβL (86B8) Rabbit mAb
- 2140 Rictor Antibody
- 2114 Rictor (53A2) Rabbit mAb
- 4978 Raptor Antibody
- 2280 Raptor (24C12) Rabbit mAb
- 2214 Raptor (W990) Antibody
- 2972 mTOR Antibody
- 2971 Phospho-mTOR (Ser2448) Antibody
- 2974 Phospho-mTOR (Ser2481) Antibody
- 9904 Rapamycin (FRAP/mTOR Inhibitor)
- 9202 p70 S6 Kinase Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
