Product Pathways - Translational Control
GβL Antibody #3227
|W IP IHC-P||H Mk||Endogenous||37||Rabbit|
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
GβL Antibody detects endogenous levels of total GβL protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using GβL Antibody in the presence of control peptide (left) or antigen specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic localization, using GβL Antibody.
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
- Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
- Ohne, Y. et al. (2008) J Biol Chem 283, 31861-70. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.