Product Pathways - Jak/Stat Pathway
Jak2 (24B11) Rabbit mAb #3229
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R B | 125 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Jak2 (24B11) Rabbit mAb detects endogenous levels of total Jak2 protein. No cross reactivity was observed with other family members.
Source / Purification
Rabbit monoclonal antibodies were prepared by immunzing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding proline 841 of Jak2.
Western Blotting
Western blot analysis of extracts from LnCap, K562 and BaF3 cell lines, using Jak2 (24B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Jak2 (24B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Jak2 (24B11) Rabbit mAb in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded U-87MG xenograft, showing cytplasmic localization, using Jak2 (24B11) Rabbit mAb.
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
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Companion Products
- 3332 Jak1 Antibody
- 3331 Phospho-Jak1 (Tyr1022/1023) Antibody
- 7752 HTScan® Jak2 Kinase Assay Kit
- 7751 Jak2 Kinase
- 3771 Phospho-Jak2 (Tyr1007/1008) Antibody
- 3774 Phospho-Jak2 (Tyr221) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 1039 Jak2 Blocking Peptide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
