Product Pathways - Jak/Stat Pathway
Jak2 (D2E12) Rabbit mAb #3230
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R (Mk) | Endogenous | 125 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Jak2 (D2E12) Rabbit mAb detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.
Source / Purification
Monoclonal antibody is produced by immunzing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro841 of Jak2.
Western Blotting
Western blot analysis of extracts from K562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of K-562 cells using Jak2 (D2E12) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of K562 cells using Jak2 (D2E12) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
- Leonard, W.J. and O'Shea, J.J. (1998) Annu. Rev. Immunol. 16, 293-322.
- Darnell, J.E. (1997) Science 277, 1630-1635.
- VanderKuur, J. et al. (1995) J. Biol. Chem. 270, 7587-7593.
- Argetsinger, L.S. et al. (1995) J. Biol. Chem. 270, 14685-14692.
- Zhu, T. et al. (1998) J. Biol. Chem. 273, 10682-10689.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3776 Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb
- 3774 Phospho-Jak2 (Tyr221) Antibody
- 7751 Jak2 Kinase
- 7752 HTScan® Jak2 Kinase Assay Kit
- 3331 Phospho-Jak1 (Tyr1022/1023) Antibody
- 3332 Jak1 Antibody
- 3775 Jak3 Antibody
- 9321 Phospho-Tyk2 (Tyr1054/1055) Antibody
- 9312 Tyk2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
