Product Pathways - Tyrosine Kinase/ Adaptors
Gab1 Antibody #3232
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 110 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Gab1 Antibody detects endogenous levels of total Gab1 protein. This antibody does not cross-react with Gab2 or Gab3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr472 of human Gab1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The GRB-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).
- Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
- Weidner, K.M. et al. (1996) Nature 384, 173-176.
- Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
- Ingham, R.J. et al. (2001) J. Biol. Chem. 276, 12257-12265.
- Lehr, S. et al. (1999) Biochemistry 38, 151-159.
- Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
- Yu, C.F. et al. (2001) J. Biol. Chem. 276, 32552-32558.
- Gual, P. et al. (2000) Oncogene 19, 1509-1518.
- Cunnick, J.M. et al. (2001) J. Biol. Chem. 276, 24380-24387.
Application References
- Guo, A. et al. (2008) Proc Natl Acad Sci U S A 105, 692-7. This article references the use of Gab1 Antibody in the following applications: Western Blotting
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Companion Products
- 3234 Phospho-Gab1 (Tyr307) Antibody
- 3231 Phospho-Gab1 (Tyr627) Antibody
- 3239 Gab2 (26B6) Rabbit mAb
- 3882 Phospho-Gab2 (Tyr452) (C33G1) Rabbit mAb
- 3881 Phospho-Gab2 (Tyr452) Antibody
- 2821 Phospho-PLCγ1 (Tyr783) Antibody
- 2822 PLCγ1 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3884 Phospho-Gab2 (Ser159) Antibody
- 3233 Phospho-Gab1 (Tyr627) (C32H2) Rabbit mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.