Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Gab1 Antibody #3232

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 110 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Gab1 Antibody detects endogenous levels of total Gab1 protein. This antibody does not cross-react with Gab2 or Gab3.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr472 of human Gab1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293, NIH/3T3-L1, C6 and COS cells, using Gab1 Antibody.

IP

IP

Immunoprecipitation of Gab 1 from insulin treated Cos cell extracts using Gab 1 antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control.

Background

The GRB-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

  1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
  2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
  3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
  4. Ingham, R.J. et al. (2001) J. Biol. Chem. 276, 12257-12265.
  5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
  6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
  7. Yu, C.F. et al. (2001) J. Biol. Chem. 276, 32552-32558.
  8. Gual, P. et al. (2000) Oncogene 19, 1509-1518.
  9. Cunnick, J.M. et al. (2001) J. Biol. Chem. 276, 24380-24387.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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