Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Phospho-Gab1 (Tyr307) Antibody #3234

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 115 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Gab1 (Tyr307) Antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 307. The antibody cross-reacts with phosphorylated Gab2 and may also cross-react with phosphorylated Gab3. The antibody may cross-react with activated receptor tyrosine kinases (RTKs).

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr307 of human Gab1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for indicated times, using Phospho-Gab1 (Tyr307) Antibody (upper) or Gab1 Antibody #3232 (lower).

Background

The GRB-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).

  1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
  2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
  3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
  4. Ingham, R.J. et al. (2001) J. Biol. Chem. 276, 12257-12265.
  5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
  6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
  7. Yu, C.F. et al. (2001) J. Biol. Chem. 276, 32552-32558.
  8. Gual, P. et al. (2000) Oncogene 19, 1509-1518.
  9. Cunnick, J.M. et al. (2001) J. Biol. Chem. 276, 24380-24387.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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