Product Pathways - Cytoskeletal Signaling
Profilin-1 Antibody #3237
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IF-IC F | H M R B | 15 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Profilin-1 Antibody detects endogenous levels of total profilin-1 protien. The antibody may cross-react with profilin-2.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human profilin-1. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using Profilin-1 Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using Profilin-1 antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent images of C2C12 cells labeled wtih Profilin-1 Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8).
- Witke, W. (2004) Trends Cell Biol 14, 461-9.
- Boukhelifa, M. et al. (2006) FEBS J 273, 26-33.
- Gareus, R. et al. (2006) J Biol Chem 281, 2803-11.
- Reinhard, M. et al. (1995) EMBO J 14, 1583-9.
- Suetsugu, S. et al. (1998) EMBO J 17, 6516-26.
- Witke, W. et al. (2001) Proc Natl Acad Sci U S A 98, 3832-6.
- Da Silva, J.S. et al. (2003) J Cell Biol 162, 1267-79.
- Hu, E. et al. (2001) Exp Nephrol 9, 265-74.
Application References
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