Product Pathways - Cytoskeletal Signaling
Caveolin-1 Antibody #3238
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H M R Pg Hm B | Endogenous | 21, 24 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Pg=Pig
Hm=Hamster
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Caveolin-1 Antibody detects endogenous levels of caveolin-1 protein. This antibody also detects caveolin-1beta (the short isoform of caveolin-1) but does not cross-react with caveolin-2 or -3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to human caveolin-1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines using Caveolin-1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using Caveolin-1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung using Caveolin-1 Antibody.
IHC-P (paraffin)
Immunohistochemical ananlysis of paraffin-embedded human colon using Caveolin-1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Caveolin-1 Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Caveolin-1 Antibody.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft showing cytoplasmic and membrane localization, using Caveolin-1 Antibody.
Flow Cytometry
Flow cytometric analysis of Hela cells using Caveolin-1 antibody (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of C2C12 cells using Caveolin-1 Antibody (green). Actin filaments have been labeled with Alexa Fluor(R) 555 phalloidin (red). Blue pseudocolor = DRAQ5(TM) (fluorescent DNA dye).
Background
The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolins family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as G-alpha subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
- Okamoto, T. et al. (1998) J. Biol. Chem. 273, 5419-5422.
- Smart, E. J. et al. (1999) Mol. Cell. Biol. 19, 7289-7304.
- Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
- Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
- Lee, H. et al. (2000) Mol. Endocrinol. 14, 1750-1775.
- Schlegel, A. et al. (2001) J. Biol. Chem. 276, 4398-4408.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3251 Phospho-Caveolin-1 (Tyr14) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
