Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Flotillin-2 (L294) Antibody #3244

Applications Reactivity MW (kDa) Source
W H M R Mk 49 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Flotillin-2 (L294) Antibody detects endogenous levels of total flotillin-2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding residue surrounding Leu294 of human flotillin-2. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Flotillin-2 (L294) Antibody.

Background

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry a evolutionarily conserved domain called the prohibitin homology domain (PHB) (1). Flotillin members are ubiquitously expressed and located to non-caveolar microdomains (lipid rafts) on the cell plasma membrane where they support signal transduction and regulate lipid raft motility and localization (2-5). Two flotillin members have been described, flotillin-1 and flotillin-2. In addition to their colocalization with lipid rafts on the plasma membrane, flotillin-1 also has been found at compartments of the endocytic and autophagosomal pathways, such as recycling/late endosomes, the Golgi complex, as well as the nucleus (6,7). Flotillin-2 is mainly localized to the plasma membrane and prevalent in cell-cell contact sites, however, overexpressed flotillin-2 has also been found in the late endosome (4,8,9). Both flotillin-1 and flotillin-2 are commonly used as lipid raft associated markers.

  1. Morrow, I.C. and Parton, R.G. (2005) Traffic 6, 725-40.
  2. Langhorst, M.F. et al. (2005) Cell Mol Life Sci 62, 2228-40.
  3. Stuermer, C.A. and Plattner, H. (2005) Biochem Soc Symp , 109-18.
  4. Fernow, I. et al. (2007) Eur J Cell Biol 86, 345-52.
  5. Neumann-Giesen, C. et al. (2007) J Cell Sci 120, 395-406.
  6. Liu, J. et al. (2005) J Biol Chem 280, 16125-34.
  7. Santamaria, A. et al. (2005) Mol Cell Biol 25, 1900-11.
  8. Solomon, S. et al. (2002) Immunobiology 205, 108-19.
  9. Neumann-Giesen, C. et al. (2004) Biochem J 378, 509-18.

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