Product Pathways - Cytoskeletal Signaling
Profilin-1 (C56B8) Rabbit mAb #3246
|3246S||100 µl (10 western blots)||---||In Stock||---|
|3246||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Dog||Endogenous||15||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
Profilin-1 (C56B8) Rabbit mAb detects endogenous levels of total profilin-1 protein. The antibody may cross-react with profilin-2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human profilin-1.
The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8).
- Witke, W. (2004) Trends Cell Biol 14, 461-9.
- Boukhelifa, M. et al. (2006) FEBS J 273, 26-33.
- Gareus, R. et al. (2006) J Biol Chem 281, 2803-11.
- Reinhard, M. et al. (1995) EMBO J 14, 1583-9.
- Suetsugu, S. et al. (1998) EMBO J 17, 6516-26.
- Witke, W. et al. (2001) Proc Natl Acad Sci U S A 98, 3832-6.
- Da Silva, J.S. et al. (2003) J Cell Biol 162, 1267-79.
- Hu, E. et al. (2001) Exp Nephrol 9, 265-74.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.