Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Profilin-1 (C56B8) Rabbit mAb #3246

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Hm Mk Dg Endogenous 15 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Profilin-1 (C56B8) Rabbit mAb detects endogenous levels of total profilin-1 protein. The antibody may cross-react with profilin-2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human profilin-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Profilin-1 (C56B8) Rabbit mAb.

Background

The dynamic polymerization and depolymerization of actin filaments, a process governed by external and internal signaling events, is vital for cell motility (immune cell function, migration, invasion, metastasis, angiogenesis), cell division and adhesion. Among the many regulators of actin dynamics are profilins. Profilins are conserved actin binding proteins that affect the rate of actin polymerization by binding actin monomers and promoting the exchange of ADP for ATP (reviewed in 1). Profilins bind to proteins involved in the regulation of actin dynamics including palladin (2), dynamin-1 (3), VASP (4) and N-WASP (5). In mice, knockout of the ubiquitously expressed profilin-1 indicates that the protein is essential for embryonic development (6). Profilin-2 is primarily expressed in brain and functions in the regulation of neurite outgrowth (7), membrane trafficking and endocytosis (3). The recently cloned profilin-3 is expressed in kidney and testes (8).

  1. Witke, W. (2004) Trends Cell Biol 14, 461-9.
  2. Boukhelifa, M. et al. (2006) FEBS J 273, 26-33.
  3. Gareus, R. et al. (2006) J Biol Chem 281, 2803-11.
  4. Reinhard, M. et al. (1995) EMBO J 14, 1583-9.
  5. Suetsugu, S. et al. (1998) EMBO J 17, 6516-26.
  6. Witke, W. et al. (2001) Proc Natl Acad Sci U S A 98, 3832-6.
  7. Da Silva, J.S. et al. (2003) J Cell Biol 162, 1267-79.
  8. Hu, E. et al. (2001) Exp Nephrol 9, 265-74.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products