Product Pathways - Cytoskeletal Signaling
Phospho-Caveolin-1 (Tyr14) Antibody #3251
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 23, 25 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Caveolin-1 (Tyr14) Antibody detects endogenous levels of caveolin-1 only when phosphorylated at tyrosine 14. The antibody does not cross-react with caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr14 of human caveolin-1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blotting analysis of extracts from HeLa cells treated with H2O2 for the indicated times, using Phospho-Caveolin-1 (Tyr14) Antibody (upper) or caveolin-1 antibody (lower).
Background
The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).
- Okamoto, T. et al. (1998) J. Biol. Chem. 273, 5419-5422.
- Smart, E. J. et al. (1999) Mol. Cell. Biol. 19, 7289-7304.
- Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986.
- Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103.
- Lee, H. et al. (2000) Mol. Endocrinol. 14, 1750-1775.
- Schlegel, A. et al. (2001) J. Biol. Chem. 276, 4398-4408.
Application References
- Salatino, M. et al. (2006) Oncogene 25, 7723-39. This article references the use of Phospho-Caveolin-1 (Tyr14) Antibody in the following applications: Western Blotting
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3238 Caveolin-1 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
