Product Pathways - Cell Cycle / Checkpoint
TTK Antibody #3255
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H (Mk) | Endogenous | 95 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
TTK Antibody detects endogenous levels of total TTK protein.
Source / Purification
Polyclonal antibodies were prepared by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Glu137 of human TTK. Antibodies were purified by affinity chromatography.
Western Blotting
Western blot analysis of recombinant GST-TTK (#7655) and extracts from OVCAR8 and K562 cell lines using TTK Antibody.
IF-IC
Confocal immunofluorescent analysis of OVCAR8 cells using TTK Antibody showing nuclear localization.
Background
TTK (Mps1, PYT) is a cell cycle regulated dual specificity kinase present in rapidly proliferating tissues and cell lines (1-3). TTK localizes to kinetochores and centromeres and is an essential component of the mitotic spindle checkpoint as well as centrosome duplication (4-6). The mitotic checkpoint inhibits entry into anaphase until all chromosomes are attached to the spindle; inhibition of this process leads to genomic instability and tumorigenesis. Phosphorylation of the BLM helicase at Ser144 by TTK maintains chromosome stability during mitosis (7). Small molecule inhibitors of TTK can block the spindle checkpoint response and TTK may present a therapeutic target (8,9).TTK also participates in the DNA damage response by directly phosphorylating and activating the cell cycle checkpoint kinase Chk2 at Thr68. Two targets phosphorylated by Chk2 are the cell cycle phosphatase cdc25 and the transcription factor p53. Inactivation of cdc25 phosphatase results in the accumulation of inactive cyclin B and cell cycle arrest following DNA damage. Phosphorylation of p53 by active Chk2 stabilizes the transcription factor and promotes cell cycle arrest and apoptosis in response to DNA damage (10).
- Mills, G.B. et al. (1992) J. Biol. Chem. 267, 16000-16006.
- Stucke, V.M. et al. (2002) EMBO J. 21, 1723-1732.
- Lindberg, R.A. et al. (1993) Oncogene 8, 351-359.
- Fisk, H.A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 14875-14880.
- Dou, Z. et al. (2003) Cell Res. 13, 443-449.
- Abrieu, A. et al. (2001) Cell 106, 83-93.
- Leng, M. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 11485-11490.
- Schmidt, M. et al. (2005) EMBO Rep. 6, 866-872.
- Dorer, R.K. et al. (2005) Curr. Biol. 15, 1070-1076.
- Wei, J.H. et al. (2005) J. Biol. Chem. 280, 7748-7757.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4718 Aurora A/AIK (1G4) Rabbit mAb
- 2661 Phospho-Chk2 (Thr68) Antibody
- 2662 Chk2 Antibody
- 4682 Mad-1 Antibody
- 4535 PLK1 Antibody
- 7655 TTK Kinase
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
