Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Blk Antibody #3262

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 55 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Blk Antibody detects endogenous levels of total Blk.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy-terminus of human Blk. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of Namalwa (lane 1) and HuT 78 (lane 2) cells grown in media with 10% FBS, using Blk Antibody.

Background

Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of Blk (3). It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5). Blk transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6). Blk function may be redundant, however, as mice that do not express Blk are not impaired with respect to B cell development and immune response (7).

  1. Dymecki, S.M. et al. (1990) Science 247, 332-336.
  2. Wasserman, R. et al. (1995) J. Immunol. 155, 644-651.
  3. Sefton, B.M. et al. (1994) Curr. Opin. Immunol. 6, 372-379.
  4. Malek, S.N. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 7351-7356.
  5. Yao, X.R. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 7946-7950.
  6. Islam, K.B. et al. (1995) J. Immunol. 154, 1265-1272.
  7. Texido, G. et al. (2000) Mol. Cell Biol. 20, 1227-1233.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products