Product Pathways - Wnt / Hedgehog / Notch
Notch1 (C44H11) Rabbit mAb #3268
Have you tried your application using our XP® monoclonal antibodies? Try product: 3608
PhosphoSitePlus® protein, site, and accession data: Notch 1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 120, 300 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3268:
- Western Blotting
Specificity / Sensitivity
Notch1 (C44H11) Rabbit mAb detects endogenous levels of total Notch1 protein. It recognizes both the full-length (~ 300 kDa) and the transmembrane/intracellular region NTM (~120 kDa).
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2439 of human Notch1.
Western Blotting
Western blot analysis of total extract from HBP-ALL cells using Notch1 (C44H11) Rabbit mAb. The full-length (FL) and transmembrane/intracellular region (NTM) are indicated.
Western Blotting
Western blot analysis of extracts from various cell lines using Notch1 (C44H11) Rabbit mAb. The transmembrane/intracellular region (NTM) is indicated.
Background
Notch1 is a transmembrane protein functioning in development and the determination of cell fate (1). During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
- Artavanis-Tsakonas, S. et al. (1999) Science 284, 770-6.
- Chan, Y.M. and Jan, Y.N. (1998) Cell 94, 423-6.
- Schroeter, E.H. et al. (1998) Nature 393, 382-6.
- Rand, M.D. et al. (2000) Mol Cell Biol 20, 1825-35.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
