Product Pathways - Glucose Metabolism
Phospho-SGK (Ser78) Antibody #3271
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 54 (Transfected only) | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-SGK (Ser78) Antibody detects transfected levels of SGK1 only when phosphorylated at serine 78. It will not detect isoforms SGK2 or SGK3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser78 of human SGK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells untransfected (left lane) and transfected by HA-SGK (right lane), using Phospho-SGK (Ser78) Antibody (upper) or control SGK Antibody #3272 (lower). The phospho-specificity of the antibody was characterized by treating the membrane with or without calf intestinal alkaline phosphatase (CIP) after Western transfer.
Background
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (2). SGK is negatively regulated by ubiquitin modification and proteasome degradation (7).
The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78 (6).
- Webster, M.K. et al. (1993) Mol. Cell. Biol. 13, 2031-2040.
- Kobayashi, T. and Cohen, P. (1999) Biochem. J. 339, 319-328.
- Park, J. et al. (1999) EMBO J. 18, 3024-3033.
- Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952-965.
- Mikosz, C.A. et al. (2001) J. Biol. Chem. 276, 16649-16654.
- Hayashi, M. et al. (2001) J. Biol. Chem. 276, 8631-8634.
- Brickley, D.R. et al. (2002) J. Biol. Chem. 277, 43064-43070.
Application References
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Companion Products
- 3272 SGK Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
