Product Pathways - Glucose Metabolism
SGK Antibody #3272
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) | Transfected Only | 54 (Transfected only) | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
SGK Antibody detects transfected levels of SGK.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the carboxy-terminal domain of human SGK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells transfected with HA-SGK using SGK Antibody.
Background
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt (1). SGK is rapidly induced in response to a variety of stimuli, including serum, glucocorticoid, follicle stimulating hormone, osmotic shock and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways (2,3). Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation (4-6). SGK also plays an important role in activating certain potassium, sodium and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion (2). SGK is negatively regulated by ubiquitin modification and proteasome degradation (7).
- Webster, M.K. et al. (1993) Mol. Cell. Biol. 13, 2031-2040.
- Kobayashi, T. and Cohen, P. (1999) Biochem. J. 339, 319-328.
- Park, J. et al. (1999) EMBO J. 18, 3024-3033.
- Brunet, A. et al. (2001) Mol. Cell. Biol. 21, 952-965.
- Mikosz, C.A. et al. (2001) J. Biol. Chem. 276, 16649-16654.
- Hayashi, M. et al. (2001) J. Biol. Chem. 276, 8631-8634.
- Brickley, D.R. et al. (2002) J. Biol. Chem. 277, 43064-43070.
Application References
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Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3271 Phospho-SGK (Ser78) Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
