Product Pathways - Translational Control
GβL (86B8) Rabbit mAb #3274
PhosphoSitePlus® protein, site, and accession data: LST8
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
GβL (86B8) Rabbit mAb detects endogenous levels of total GβL protein.
Source / Purification
GβL (86B8) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gln210 of human GβL.
Western Blotting
Western blot analysis of extracts from GβL wild-type (+/+) and knockout (-/-) mouse embryonic fibroblast cells using GβL (86B8) Rabbit mAb. (provided by David Sabatini Lab at the Whitehead Institute for Biomedical Research).
Western Blotting
Western blot analysis of extracts from various cell lines, using GβL (86B8) Rabbit mAb.
Background
Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
- Sarbassov, D.D. et al. (2004) Curr Biol. 14, 1296-1302.
- Kim, D.H. et al. (2003) Mol. Cell 11, 895-904.
Application References
- Wu, M. et al. (2010) Rejuvenation Res 13, 571-9. Applications: Western Blotting
- Gao, D. et al. (2011) Mol Cell 44, 290-303. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
