Product Pathways - Protein Folding/Stability
HAUSP Antibody #3277
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 135 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
HAUSP Antibody detects endogenous levels of total HAUSP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Asp438 of human HAUSP. Antibodies are purified by peptide affinity chromatography.
Western blot analysis of extracts from MCF-7, NIH/3T3 and PC12 cells using HAUSP Antibody.
Background
Ubiquitinating enzymes (UBEs) catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme (DUB) action (1,2). Five DUB subfamilies are recognized, including the USP, UCH, OTU, MJD and JAMM enzymes. HAUSP (USP7 or herpesvirus-associated ubiquitin-specific protease) is an important deubiquitinase belonging to USP subfamily. A key HAUSP function HAUSP is to bind and deubiquinate the p53 transcription factor and an associated regulator protein Mdm2, thereby stabilizing both proteins (3,4). In addition to regulating essential components of the p53 pathway, HAUSP also modifies other ubiquitinylated proteins such as members of the FoxO family of forkhead transcription factors and the mitotic stress checkpoint protein CHFR (5,6).
- Nijman, S.M. et al. (2005) Cell 123, 773-86.
- Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
- Li, M. et al. (2002) Nature 416, 648-53.
- Brooks, C.L. et al. (2007) Oncogene (Epub ahead of print).
- van der Horst, A. et al. (2006) Nat Cell Biol 8, 1064-73.
- Oh, Y.M. et al. (2007) Biochem Biophys Res Commun 357, 615-9.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
