Cell Signaling Technology

Product Pathways - Neuroscience

Shootin1 Antibody #3279

Applications Reactivity MW (kDa) Source
W IP H M R 60 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Shootin1 Antibody detects endogenous levels of total shootin1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) surrounding Lys450 of human shootin1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from neonatal mouse and fetal rat brain using Shootin1 Antibody.

Background

The differentiation process of neurons can be divided into five stages, each stage characterized by morphological changes observed in the developing cells. In stage 1, the cells extend lamellipodia and in stage 2 their lamellipodia develop into immature neurites. In stage 3 one neurite elongates rapidly to form an axon and in stage 4 the remaining immature neuritis elongate to form dendrites. In stage 5 synaptic contacts are formed and a neuronal network is established (1).Shootin1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. The extension of an axon requires considerable reorganization of the cytoskeleton mediated by PI3K/Akt and PI3K/Cdc42 signaling (1). Shootin1 is involved in regulating the subcellular localization of PI3 kinase. Furthermore, shootin1 is upregulated during polarization and accumulates asymmetrically in a single neurite that consequently elongates rapidly to form an axon (2).

  1. Yoshimura, T. et al. (2006) J Neurosci 26, 10626-30.
  2. Toriyama, M. et al. (2006) J Cell Biol 175, 147-57.

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