Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Orai1 (D90B10) Rabbit mAb #3280

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 32 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Orai1 (D90B10) Rabbit mAb detects endogenous levels of total Orai1 protein. The antibody also cross-reacts with an unidentified protein at 78 kDa.

Source / Purification

Monolonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) derived from the sequence of mouse Orai1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Orai1 (D90B10) Rabbit mAb.

Background

Calcium is a key second messenger in many intracellular signaling pathways. Ca2+ signals control many cellular functions ranging from short term responses such as contraction and secretion to longer term regulation of cell growth and proliferation (1,2). Orai1 is the Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1 (3). The STIM1 and Orai1 are required for functional CRAC current and their co-expression leads to greatly amplified CRAC currents (4-7). There are three human homologues, Orai1, Orai2 and Orai3, which have been shown to be capable of forming store-operated channels (3, 5, 8). The Orai1, -2, and -3 channels are similarly inhibited by extracellular Ca2+, and Orai3 channels appeared to differ from Orai1 and -2 in being somewhat resistant to the process of Ca2+ depotentiation (9).

  1. Berridge, M.J. et al. (2000) Nat Rev Mol Cell Biol 1, 11-21.
  2. Berridge, M.J. et al. (2003) Nat Rev Mol Cell Biol 4, 517-29.
  3. Vig, M. et al. (2006) Science 312, 1220-3.
  4. Zhang, S.L. et al. (2006) Proc Natl Acad Sci USA 103, 9357-62.
  5. Mercer, J.C. et al. (2006) J Biol Chem 281, 24979-90.
  6. Peinelt, C. et al. (2006) Nat Cell Biol 8, 771-3.
  7. Soboloff, J. et al. (2006) J Biol Chem 281, 20661-5.
  8. Vig, M. et al. (2006) Curr Biol 16, 2073-9.
  9. DeHaven, W.I. et al. (2007) J Biol Chem 282, 17548-56.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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