Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-FAK (Tyr576/577) Antibody #3281
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) (R) (C) (X) | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
C=Chicken
X=Xenopus
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-FAK (Tyr576/577) Antibody detects endogenous levels of FAK only when phosphorylated at tyrosine 576/577.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr576/577 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower).
Background
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of tyrosine residues at 407, 576 and 577 in the catalytic domain, and tyrosine residues at 871 and 925 in the carboxy-terminal region of FAK (6,7).
Tyr576 and Tyr577 lie in the activation loop of the kinase domain, and mutation of these residues reduces FAK catalytic activity (6).
- Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
- Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
- Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
- Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
- Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
- Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
- Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.
Application References
- John, G. R. et al. (2004) Interleukin-1beta Induces a Reactive Astroglial Phenotype via Deactivation of the Rho GTPase-Rock Axis. The Journal of Neuroscience 24 (11), 2837-2845. This article references the use of Phospho-FAK (Tyr576/577) Antibody in the following applications: IC-IF Western Blotting
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Companion Products
- 3284 Phospho-FAK (Tyr925) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3285 FAK Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
