Product Pathways - Tyrosine Kinase / Adaptors
Phospho-FAK (Tyr397) Antibody #3283
PhosphoSitePlus® protein, site, and accession data: FAK
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Hm Pg | Endogenous | 125 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3283:
- Western Blotting
Specificity / Sensitivity
Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untransfected or transfected with activated Src, using Phospho-FAK (Tyr397) Antibody (upper) or FAK Antibody #3285 (lower).
Background
Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).
- Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
- Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
- Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
- Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
- Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
- Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
- Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.
Application References
- Zaytseva, Y.Y. et al. (2012) Cancer Res 72, 1504-17. Applications: IF-IC (In Cells) Western Blotting
- Fu, X.D. et al. (2010) Endocr Relat Cancer 17, 431-43. Applications: Western Blotting
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Companion Products
- 3285 FAK Antibody
- 3281 Phospho-FAK (Tyr576/577) Antibody
- 3284 Phospho-FAK (Tyr925) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
