Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Phospho-FAK (Tyr397) Antibody #3283

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC F H M Pg Hm (R) Endogenous 125 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Pg=Pig  Hm=Hamster
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated EGF receptors.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Tyr397 of human FAK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untransfected or transfected with activated Src, using Phospho-FAK (Tyr397) Antibody (upper) or FAK Antibody #3285 (lower).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, phosphatase-treated (red), untreated (blue) or H2O2-treated (green), using Phospho-FAK (Tyr397) Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 cells, untreated (left) or lambda phosphatase-treated (right), using Phospho-FAK (Tyr397) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the carboxy-terminal region of FAK (6,7).

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
  2. Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
  3. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
  4. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
  5. Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
  6. Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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