Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Endoglin Antibody (Mouse Specific) #3290

Applications Reactivity Sensitivity MW (kDa) Source
W M Transfected Only 79, 81 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Endoglin Antibody (Mouse Specific) detects transfected levels of mouse endoglin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro430 of mouse endoglin. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with mouse endoglin, using Endoglin Antibody (Mouse Specific).

Background

Endoglin (ENG, CD105) is an auxiliary receptor for the TGF-β receptor complex, functioning in related signaling pathways (1,2). Endoglin is a transmembrane protein that exists as a disulfide-linked homodimer. It is mainly expressed in vascular and connective tissues and in endothelial and stromal cells. Upregulated endoglin expression has been reported during wound healing and tumor vascularization, and in inflammatory tissues and developing embryos (1-4). Mutations in endoglin have been found to be a causal factor of hereditary hemorrhagic telangiectasia (HHT), a disease characterized by malformation of vascular structure (5,6). The importance of this protein for normal and tumor vascular function makes it a good marker for endothelial cell proliferation as well as a potential therapeutic target in cancer (4-6).

  1. Bernabeu, C. et al. (2007) J Cell Biochem 102, 1375-88.
  2. Zhu, Y. et al. (2003) Stroke 34, 2483-8.
  3. Quintanilla, M. et al. (2003) Oncogene 22, 5976-85.
  4. Dallas, N.A. et al. (2008) Clin Cancer Res 14, 1931-7.
  5. ten Dijke, P. et al. (2008) Angiogenesis 11, 79-89.
  6. Fernández-L, A. et al. (2006) Clin Med Res 4, 66-78.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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