Product Pathways - Tyrosine Kinase/ Adaptors

Pyk2 Antibody #3292

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M	Endogenous	116	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Pyk2 Antibody detects endogenous levels of total Pyk2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding the amino-terminus of human Pyk2. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Protein tyrosine kinase Pyk2, also called CAKbeta, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).

1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
2. Lev, S. et al. (1995) Nature 376, 737-745.
3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.

Application References

• McMullen, M. et al. (2004) Vascular endothelial growth factor-mediated activation of p38 is dependent upon Src and RAFTK/Pyk2. Oncogene 23, 1275-1282. This article references the use of Pyk2 Antibody in the following applications: Western Blotting

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.