Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

SPHK1 Antibody #3297

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SPHK1 Antibody detects endogenous levels of total SPHK1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human SPHK1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SPHK1 Antibody.

Background

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

  1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
  2. Xia, P. et al. (2000) Curr Biol 10, 1527-30.
  3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
  4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
  5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
  6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
  7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
  8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
  9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
  10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
  11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
  12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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