Product Pathways - Translational Control
Phospho-GCN2 (Thr898) Antibody #3301
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 220 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 3301:
- Western Blotting
Specificity / Sensitivity
Phospho-GCN2 (Thr898) Antibody detects endogenous levels of GCN2 only when phosphorylated at threonine 898.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr898 of murine GCN2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from SV-T2 cells, untreated or UV-treated (50 mJ/cm2 for 30 minutes), using Phospho-GCN2 (Thr898) Antibody.
Background
Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (1,2). GCN2 is also required for UV-light induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-kappaB also requires GCN2, which may act simply by preventing translation of IkappaB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6).
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-6128.
- Deng, J. et al. (2002) Curr. Biol. 12, 1279-1286.
- Jiang, H.Y. and Wek, R.C. (2005) Biochem. J. 385, 371-380.
- Jiang, H.Y. and Wek, R.C. (2005) J. Biol. Chem. XXX, XXX-XXX.
- Garcia-Barrio, M. et al. (2002) J. Biol. Chem. 277, 30675-30683.
Application References
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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
