Product Pathways - Translational Control
GCN2 Antibody #3302
|W IP||H M R Mk||Endogenous||220||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
GCN2 Antibody detects endogenous levels of GCN2 protein independent of phosphorylation.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequence near the amino terminus of human GCN2. Antibodies are purified by protein A and peptide affinity chromatography.
Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (1,2). GCN2 is also required for UV-light induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-kappaB also requires GCN2, which may act simply by preventing translation of IkappaB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6).
- Kaufman, R.J. (1999) Genes Dev. 13, 1211-1233.
- Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-6128.
- Deng, J. et al. (2002) Curr. Biol. 12, 1279-1286.
- Jiang, H.Y. and Wek, R.C. (2005) Biochem. J. 385, 371-380.
- Jiang, H.Y. and Wek, R.C. (2005) J. Biol. Chem. XXX, XXX-XXX.
- Garcia-Barrio, M. et al. (2002) J. Biol. Chem. 277, 30675-30683.
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For Research Use Only. Not For Use In Diagnostic Procedures.