Cell Signaling Technology

Product Pathways - Protein Folding

HSPA4/Apg-2 Antibody #3303

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 110 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HSPA4/Apg-2 Antibody detects endogenous levels of total HSPA4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human HSPA4. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HSPA4/Apg-2 Antibody.

Background

The heat shock protein HSPA4 (Apg-2, HSP70RY) is a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 heat-shock proteins (1). Apg-2 has chaperone ability similiar to Hsp110, and it plays a role under non-stress conditions (2). Apg-2 interacts with TJP1/ZO-1 and functions as a regulator of ZO-1-ZONAB signaling in epithelial cells in response to cellular stress (3,4).

  1. Kaneko, Y. et al. (1997) Gene 189, 19-24.
  2. Gotoh, K. et al. (2004) FEBS Lett 560, 19-24.
  3. Tsapara, A. et al. (2006) Mol Biol Cell 17, 1322-30.
  4. Aijaz, S. et al. (2007) BMC Cell Biol 8, 49.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products