Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

VRK1 (1F6) Mouse mAb #3307

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 48 Mouse IgG1

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

VRK1 (1F6) Mouse mAb detects endogenous levels of total VRK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant fusion protein GST-full length human VRK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells using VRK1 (1F6) Mouse mAb.

Background

The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses Erk activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation.

  1. Nezu, J. et al. (1997) Genomics 45, 327-31.
  2. Zelko, I. et al. (1998) Arch Biochem Biophys 352, 31-6.
  3. Vega, F.M. et al. (2003) FEBS Lett 544, 176-80.
  4. Lopez-Borges, S. and Lazo, P.A. (2000) Oncogene 19, 3656-64.
  5. Sevilla, A. et al. (2004) J Biol Chem 279, 27458-65.
  6. Sevilla, A. et al. (2004) Oncogene 23, 8950-8.
  7. Nichols, R.J. and Traktman, P. (2004) J Biol Chem 279, 7934-46.
  8. Kang, T.H. and Kim, K.T. (2006) Nat Cell Biol 8, 863-9.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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