Product Pathways - Cytoskeletal Signaling
Phospho-Cofilin (Ser3) Antibody #3311
PhosphoSitePlus® protein, site, and accession data: cofilin 1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Hm Mk B (Pg) | Endogenous | 19 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Hm=Hamster
Mk=Monkey
B=Bovine
Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3311:
- Western Blotting
Specificity / Sensitivity
Phospho-Cofilin (Ser3) Antibody detects endogenous levels of cofilin only when phosphorylated at serine 3. The antibody may cross-react with phosphorylated cofilin 2, the muscle isoform.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or H2O2-treated, using Phospho-Cofilin (Ser3) Antibody (A and B) or Cofilin Antibody #3312 (C and D). Membranes (B and D) were treated with alkaline phosphatase (CIP) to demonstrate the phospho-specificity the antibody.
Western Blotting
Western blot analysis of extracts from asynchronous or mitotic C6, CHO and COS cells, using Phospho-Cofilin (Ser3) Antibody (upper) or Cofilin Antibody #3312 (lower).
Background
Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
- Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
- Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
- Arber, S. et al. (1998) Nature 393, 805-9.
- Yang, N. et al. (1998) Nature 393, 809-12.
- Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
- Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.
Application References
- Melendez-Vasquez, C.V. et al. (2004) J Neurosci 24, 3953-63. Applications: Western Blotting
- Wabnitz, G.H. et al. (2006) J Immunol 176, 1668-74. Applications: Western Blotting
- Hsieh, S.H. et al. (2006) J Neurosci 26, 1006-15. Applications: Western Blotting
- Goeckeler, Z.M. and Wysolmerski, R.B. (2005) J Biol Chem 280, 33083-95. Applications: Western Blotting
- Chen, X. and Macara, I.G. (2006) J Cell Biol 172, 671-8. Applications: Western Blotting
- Misra, U.K. et al. (2005) J Immunol 175, 2525-33. Applications: Western Blotting
- Kamanova, J. et al. (2008) J Immunol 181, 5587-97. Applications: Western Blotting
- Thirone, A.C. et al. (2009) Am J Physiol Cell Physiol 296, C463-75. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3312 Cofilin Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3313 Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
- 3318 Cofilin (D59) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
