Product Pathways - Cytoskeletal Signaling
Phospho-Cofilin (Ser3) Antibody #3311
|W||H M R Hm Mk B (Pg)||Endogenous||19||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Phospho-Cofilin (Ser3) Antibody detects endogenous levels of cofilin only when phosphorylated at serine 3. The antibody may cross-react with phosphorylated cofilin 2, the muscle isoform.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from HeLa cells, untreated or H2O2-treated, using Phospho-Cofilin (Ser3) Antibody (A and B) or Cofilin Antibody #3312 (C and D). Membranes (B and D) were treated with alkaline phosphatase (CIP) to demonstrate the phospho-specificity the antibody.
Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
- Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
- Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
- Arber, S. et al. (1998) Nature 393, 805-9.
- Yang, N. et al. (1998) Nature 393, 809-12.
- Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
- Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.
- Melendez-Vasquez, C.V. et al. (2004) J Neurosci 24, 3953-63. Applications: Western Blotting
- Wabnitz, G.H. et al. (2006) J Immunol 176, 1668-74. Applications: Western Blotting
- Hsieh, S.H. et al. (2006) J Neurosci 26, 1006-15. Applications: Western Blotting
- Goeckeler, Z.M. and Wysolmerski, R.B. (2005) J Biol Chem 280, 33083-95. Applications: Western Blotting
- Chen, X. and Macara, I.G. (2006) J Cell Biol 172, 671-8. Applications: Western Blotting
- Misra, U.K. et al. (2005) J Immunol 175, 2525-33. Applications: Western Blotting
- Kamanova, J. et al. (2008) J Immunol 181, 5587-97. Applications: Western Blotting
- Thirone, A.C. et al. (2009) Am J Physiol Cell Physiol 296, C463-75. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.