Product Pathways - Cytoskeletal Signaling
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H M R Mk B | Endogenous | 19 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.
Western Blotting
Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Coflin (Ser3) (77G2) Rabbit mAb #3313.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells either untreated (left) or lambda-phosphatase treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
- Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830.
- Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
- Arber, S. et al. (1998) Nature 393, 805-809.
- Yang, N. et al. (1998) Nature 393, 809-812.
- Toshima, J. et al. (2001) J. Biol. Chem. 276, 31449-31458.
- Nebl, G. et al. (1996) J. Biol. Chem. 271, 26276-26280.
Application References
- Negash, S. et al. (2009) Am J Physiol Heart Circ Physiol , . Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3311 Phospho-Cofilin (Ser3) Antibody
- 3312 Cofilin Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3318 Cofilin (D59) Antibody
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
