Product Pathways - Cytoskeletal Signaling
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313
|3313L||300 µl (30 western blots)||---||In Stock||---|
|3313S||100 µl (10 western blots)||---||In Stock||---|
|3313P||40 µl (4 western blots)||---||In Stock||---|
|3313||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey, Bovine||Endogenous||19||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.
Western blot analysis of COS cells, untreated or λ phosphatase-treated, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (upper) or Cofilin Antiobdy #3312 (lower).
Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.
Confocal immunofluorescent analysis of MCF7 cells either untreated (left) or λ phosphatase-treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
- Negash, S. et al. (2009) Am J Physiol Heart Circ Physiol , . Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.