Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb #3313

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC H M R Mk B Endogenous 19 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Cofilin (Ser3) (77G2) Rabbit mAb detects endogenous levels of cofilin only when phosphorylated at serine 3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser3 of human cofilin.

Western Blotting

Western Blotting

Western blot analysis of COS cells, untreated or λ phosphatase-treated, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (left) or Cofilin Antiobdy #3312 (right).

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, λ phosphatase-treated or untreated, and various other cell lines, using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF7 cells either untreated (left) or λ phosphatase-treated (right), using Phospho-Cofilin (Ser3) (77G2) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).

  1. Carlier, M.F. et al. (1999) J Biol Chem 274, 33827-30.
  2. Condeelis, J. (2001) Trends Cell Biol 11, 288-93.
  3. Arber, S. et al. (1998) Nature 393, 805-9.
  4. Yang, N. et al. (1998) Nature 393, 809-12.
  5. Toshima, J. et al. (2001) J Biol Chem 276, 31449-58.
  6. Nebl, G. et al. (1996) J Biol Chem 271, 26276-80.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

For Research Use Only. Not For Use In Diagnostic Procedures.

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