Product Pathways - MAPK Signaling
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 49 | Rabbit IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr222.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr222 of human MAPKAPK-2.
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper) or MAPKAPK-2 Antibody #3042 (lower).
Western Blotting
Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).
Background
In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).
Application References
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Companion Products
- 3044 Phospho-MAPKAPK-2 (Thr222) Antibody
- 3042 MAPKAPK-2 Antibody
- 3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb
- 3041 Phospho-MAPKAPK-2 (Thr334) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
