Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb #3316

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 49 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at Thr222.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr222 of human MAPKAPK-2.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper) or MAPKAPK-2 Antibody #3042 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).

Background

In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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